I am trying to run a qblast from the Python prompt and after importing all the libraries I need, Python cannot find my file:
>>> record = SeqIO.read(open("sinchimeras_1.fasta"), format="fasta")
Traceback (most recent call last):
File "<stdin>", line 1, in <module>
IOError: [Errno 2] No such file or directory: 'sinchimeras_1.fasta'
I have tried to write down all the route for the file ("/Users/imac...") and to move the file to the Python and to the Biopython folders, and I get the same message.
Where do I have to save my file? What am I doing wrong?